Table 1.

The experimental design using CyDye DIGE fluor minimal dyes

Gel number	Cy2	Cy5	Cy3
1	Pooled standard	Control 1	Treated 1
2	Pooled standard	Treated 1	Control 1
3	Pooled standard	Control 2	Treated 2
4	Pooled standard	Treated 2	Control 2

DIGE, difference gel electrophoresis.

The Controls are non-pregnancy serum samples and Treated samples for pregnant serum of day 21 Holstein dairy cattle after artificial insemination. The pooled standards are mixture of pregnant and non-pregnant serum samples for internal standard.