Table 1. Summary of hMSCs sources, cell surface markers and expansion media with serum supplements.

Source	Method of isolation	Media	Serum supplement	Cell surface markers		References
Bone marrow	Ficoll density gradient method Novel marrow filter device	DMEM DMEM-F12 ADMEM	FBS	Positive	Negative	[10,36,38,47,48]
				CD73, CD90, CD105, STRO-1	CD14, CD34, CD45, HLA-DR
Adipose tissue	Digestion method Membrane filtration method	DMEM DMEM-LG	FBS FCS	CD73, CD090, CD29, CD44, CD71, CD105, CD13, CD166, STRO-1	CD14, CD31, CD34, CD45	[13,34,43,49,57]
Amniotic fluid and membrane	Density gradient method Digestion method	α-MEM DMEM/F12	FBS	CD29, CD44, CD90, CD105, CD, SH2, SH3, HLA-DR	CD10, CD14,CD34, HLA-DR	[15–17]
Dental tissues	Digestion method	α-MEM MEM	FCS FBS	CD29, CD44, CD90, CD105	CD14, CD34, CD45	[18,19,46]
Endometrium	Digestion method	DMEM-F12	FCS	CD73, CD90, CD105, CD146	CD34, CD45	[20]
Limb bud	Digestion method	DMEM-LG	FBS	CD13, CD29,CD90, CD105, CD106	CD3, CD4, CD14, CD15, CD34, CD45, HLA-DR	[21]
Peripheral blood	Ficoll density gradient	α-MEM	NBCS	CD44, CD90, CD105, HLA-ABC	CD45, CD133	[23]
Placenta and fetal membrane	Digestion method	DMEM-LG	FBS	CD29, CD73, CD90, CD105	CD34, CD45	[24]
Salivary gland	Digestion method (Ringer solution)	DMEM	FCS	CD13, CD29, CD44, CD90, STRO-1	CD34, CD45	[25]
Skin and foreskin	Digestion method	DMEM-HG DMEM DMEM-F12	FBS	CD44, CD73, CD90, CD105, CD166, SSEA-4, Vimentin	CD34, CD45, HLA-DR	[26,27]
Sub amniotic umbilical cord lining membrane	Digestion method	DMEM-HG DMEM CMRL1660	FBS	CD29, CD44, CD73, CD90, CD105	CD34, CD45	[13,28,58]
Synovial fluid	Ficoll density gradient method	α-MEM	FBS	CD44, CD90, CD105, CD147, STRO-1	CD31, CD34, CD45, CD106	[29]
Wharton's jelly	Enzymatic digestion method	DMEM	FBS	CD73, CD90, CD105	CD14, CD34, CD45, CD79, HLA-DR	[31,32]