Extended Data Figure 7. Validation of the subclonal hierarchies in A21.

Validation strategy as described in Extended Data Figure 2. Hierarchical clustering of the VAF was performed separately for substitutions (a) and indels (b). Heatmaps are annotated as described in Extended Data Figure 2. Loci with depth <20X is coloured in light blue. Additional samples L, N, and Q from FFPE material had low coverage. The only loci present in these samples were all truncal. These samples are incorporated into the phylogenetic tree (c). Numbers of substitutions in WGS data assigned to each subclone are plotted in (d). Number of indels (e) and rearrangements (f) present in different subsets of samples are plotted as bar charts. VAFs from WGS and validation data, plotted as scatter plots (g), are very highly correlated. Subclone Colour key (h).