Table 1.
Toxin profile (mol%) from the Gymnodinium catenatum culture isolated from Cascais Bay in 2007, a seawater sample collected during a bloom of G. catenatum off Aveiro in 2007 and a strain isolated from Aguda in 1989 that was cultured and analyzed by [16].
| Toxin | Toxin Cell Quota (fmol/Cell) ^ | Toxin Profile (mol%) | |||
|---|---|---|---|---|---|
| This Study | This Study | Negri et al. (2007) | |||
| Culture | Seawater | Culture | Seawater | Culture (1989) | |
| C1 + 2 | 6.5 | 4.3 | 21.0 | 22.1 | 14.5 |
| C3 + 4 | 0.5 | 0.4 | 1.6 | 2.0 | 6.0 |
| GTX2 + 3 | nd | nd | nd | nd | 1.0 |
| GTX5 | 3.4 | 6.9 | 11.0 | 35.2 | 12.9 |
| GTX6 | 1.2 | nd | 3.8 | nd | 5.9 |
| dcGTX2 + 3 | 0.3 | nd | 0.8 | nd | 4.0 |
| dcSTX | 2.2 | nd | 7.2 | nd | 2.5 |
| dcNeo | 1.1 | nd | 3.4 | nd | nd |
| STX | nd | nd | nd | nd | 0.4 |
| GC1 + 2 | 5.9 | 6.2 | 19.0 | 31.6 | 35.3 |
| GC3 | 5.0 | 1.6 | 16.2 | 8.3 | 17.6 |
| GC4 * + 5 | 1.7 | 0.2 | 5.6 | 0.8 | na |
| GC6 | 3.2 | nd | 10.3 | nd | na |
nd: not detected; na: not analyzed; * trace level GC4 detected, but not in the quantifiable range; ^ LC-MS/MS peak area for individual PSTs was compared to the corresponding toxin standard curves to calculate the concentration (µM), then standardized to the combined total fraction as a percentage to calculate the mol% as detailed in the Experimental Section.