Table 3.

MTT assay results on K562 cells for the G59 strain and its mutant samples at 100 μg/mL ^a.

Strain	Condition for Treatment of the G59 Spores at 4 °C to Select the Mutant			Inhibition Rate (IR%) at 100 μg/mL
	DMSO% (v/v)	Neomycin (mg/mL)	Treatment Time (Day)	First	Second	Third	Mean ± SD
G59	–	–	–	4.8	3.9	7.9	5.5 ± 2.1
1-50-1	20	2	15	39.8	35.3	45.5	40.2 ± 5.1
2-2	33	3.3	10	27.7	47.6	30.9	35.4 ± 10.7
2-2-3	33	3.3	10	27.0	34.5	34.6	32.0 ± 4.4
2-50-1	33	3.3	15	32.6	31.4	19.8	27.9 ± 7.1
2-s-2	33	3.3	60	47.7	73.2	38.3	53.1 ± 18.1
2-s-3	33	3.3	60	46.0	59.2	36.0	47.1 ± 11.6
3-4-1	50	5.0	5	34.5	35.2	44.7	38.1 ± 5.7
3-4-2	50	5.0	5	30.8	31.5	41.8	34.7 ± 6.2
3-50-1	50	5.0	10	43.5	27.7	53.1	41.4 ± 12.8
3-50-2	50	5.0	10	69.2	31.7	40.6	47.2 ± 19.6
3-f-1	50	5.0	15	24.8	53.4	40.3	39.5 ± 14.3
3-f-3	50	5.0	15	31.6	33.8	31.5	35.2 ± 1.3
3-f-31	50	5.0	15	55.1	29.9	38.5	41.2 ± 12.8
3-v-1	50	5.0	30	30.4	35.3	44.9	36.9 ± 7.4
3-x-1	50	5.0	30	40.3	70.1	49.7	53.4 ± 15.2
3-s-1	50	5.0	60	24.0	24.7	34.5	27.7 ± 5.9
3-s-2	50	5.0	60	55.1	23.1	49.8	42.7 ± 17.2
4-4	67	6.7	5	42.5	34.3	30.7	35.8 ± 6.0
4-16-2	67	6.7	7	29.4	32.8	29.1	30.4 ± 2.1
4-30	67	6.7	7	50.4	53.1	36.1	46.5 ± 9.1
4-x-1	67	6.7	10	47.3	34.9	51.8	44.7 ± 8.8
4-v-2	67	6.7	10	34.9	38.6	38.9	37.5 ± 2.2
4-s-1	67	6.7	15	68.1	51.4	27.5	49.0 ± 20.4
PDN-10-2	100	10	1	41.3	52.4	23.8	39.2 ± 14.4
PDN-50-1	100	10	5	52.8	25.8	47.2	41.9 ± 14.2
PDN-v-1	100	10	7	29.3	22.9	26.0	26.1 ± 3.2
PDN-v-2	100	10	7	51.2	21.8	36.1	36.4 ± 14.7
PDN-s-2	100	10	10	38.8	35.4	32.2	35.5 ± 3.3

^a The IR% values given in this table were from the triplicate MTT tests that were carried out using the EtOAc extracts from three rounds of individual fermentations of the parent G59 strain and the 28 mutants, respectively. The K562 cells were treated with the sample at 37 °C for 24 h and then the IR% was measured by the MTT method.