Table 6.
Composition of spore suspensions in Groups I–V for treatment of G59 spores at 4 °C a.
| Group | Concentration of DMSO and Neo in Test Group | Mixed Volume (mL) of Solution or Solvent with G59 Spore Suspension for I–IV Groups | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Test Group | Neo Control | DMSO Control | Blank Control | |||||||
| DMSO% | Neo mg/mL | Neo-D | G59-S | Neo-H | G59-S | DMSO | G59-S | H2O | G59-S | |
| I | 20% | 2.0 | 0.6 | 2.4 | 0.6 | 2.4 | 0.6 | 2.4 | 0.6 | 2.4 |
| II | 33% | 3.3 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 |
| III | 50% | 5.0 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 |
| IV | 67% | 6.7 | 2.0 | 1.0 | 2.0 | 1.0 | 2.0 | 1.0 | 2.0 | 1.0 |
| V | 100% | 10.0 | Approx. eq. amt. of G59 spores in each 3.0 mL of Neo-D, Neo-H, DMSO and H2O. | |||||||
a Neo is the abbreviation of neomycin. Neo-D: The neomycin solution in DMSO at 10.0 mg/mL; Neo-H: The neomycin solution in H2O at 10.0 mg/mL; G59-S: Fresh G59 spore suspensions in water (Section 3.3.1). H2O indicates sterilized-distilled water, and DMSO indicates fresh DMSO.