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. 2015 Apr 22;13(4):2505–2525. doi: 10.3390/md13042505

Figure 3.

Figure 3

Effects of xyloketal B on p-ERK, t-ERK, p-Akt, and t-Akt protein expressions. U251 cells were treated with xyloketal B (150 and 300 μM) for 24 h, and then the protein expression profile was detected by western blots. (A) Representative images of western blotting results; (B) Xyloketal B (300 μM) treatment significantly reduced p-Akt protein expression. * p < 0.05, n = 5; (C) Xyloketal B did not significantly alter the t-Akt protein expression; (D) Ratio of p-Akt/t-Akt decreased in the xyloketal B (300 μM) treatment group. * p < 0.05, n = 5; (E) Xyloketal B (300 μM) treatment significantly reduced p-ERK1/2 protein expression. * p < 0.05, n = 5; (F) Xyloketal B did not significantly alter t-ERK1/2 protein expression; (G) Ratio of p-ERK1/2/t-ERK1/2 decreased in xyloketal B (300 μM) treatment group. * p < 0.05, n = 5.