Skip to main content
. 2015 Apr 22;13(4):2505–2525. doi: 10.3390/md13042505

Figure 4.

Figure 4

Effects of xyloketal B on TRPM7 currents in HEK-293 cell over-expressing TRPM7. (A) Xyloketal B did not significantly regulate TRPM7 protein expressions in U251 cells. U251 cells were treated with xyloketal B (150 and 300 μM) for 24 h followed by detection with western blotting. n = 4; (B) Xyloketal B (300 μM) blocked TRPM7 currents. TRPM7 protein overexpression in HEK-293 cells was induced by treatment with tetracycline (Tet, 1 μg/mL) for 24 h. Then, TRPM7 currents were recorded using the whole-cell patch-clamp technology with ramp from −100 mV to 100 mV. Representative I–V traces were shown. n = 3; (C) Representative time course of the inward and outward current of TRPM7 at −100 and 100 mV. n = 3; (D) Statistical analysis of patch-clamp experiments. Xyloketal B (300 μM) perfusion significantly reduced the outward current of TRPM7. * p < 0.05, n = 3.