Fig. 5.

The atheroprotective B-cell response induced by apoptotic cells is similar to that seen in aged Apoe^−/− mice. Spleens of noninjected (−) and apoptotic cell-injected (×6) Apoe^−/− and C57BL/6 (WT) mice were investigated for MZB (A) and activated B cells (B); GC B cells (Left), and AFC (Right) by flow cytometry. In WT mice, T15 idiotype-positive AFC were assessed (C), and anti-oxLDL IgM and IgG was measured by ELISA (D). Median and individual mice (n = 5–12) are plotted (A–D). Mean and individual mice (n = 14–18) are plotted. Data shown from one representative of two experiments, n = 10 (C), representative of two, n = 5–12 (A, B, and D). *P < 0.05, **P < 0.01, and ***P < 0.001 by a Mann–Whitney U test.