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. 2015 Apr 6;112(16):5219–5224. doi: 10.1073/pnas.1417838112

Fig. 3.

Fig. 3.

Octopamine mediates starvation-induced hyperactivity. (A and C) Midline crossing activity of indicated genotype assayed in the presence and absence of 5% sucrose (n = 29–49). (B and D) Average daily midline crossing activity of flies assayed in A and C. (E–G) Midline crossing activity of indicated genotype and environmental temperature, assayed in the presence and absence of 5% sucrose (n = 56–80). Note that for G, low environmental temperature caused a late onset of starvation-induced hyperactivity. (H) Average daily midline crossing activity of flies assayed in E–G. (I) Midline crossing activity of indicated genotype and environmental temperature, assayed in the presence of 5% sucrose (n = 63–76). Gray shade indicates nonpermissive temperature (27 °C). (J) Average daily midline crossing activity of flies assayed in I, before (red) and after (blue) the temperature shift. Error bars represent SEM. Two-way ANOVA (B, D, F, H, and J) was applied to test the effect of two independent variables (genotype vs. starvation/temperature) on fly locomotion, and statistical significance was identified for both variables (B, D, F, H, and J; P < 0.0001). Post hoc multiple comparisons were then performed. NS, P > 0.05; ****P < 0.0001.