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. 2015 Apr 6;112(16):5183–5188. doi: 10.1073/pnas.1500989112

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Fig. 4. — Prevention of cellulose production restores intramacrophage replication to an mgtC null mutant. (A) Replication within J774A.1 macrophages of WT (14028s), mgtC (EL4), bcsA (MP140), bcsA mgtC (MP142), bcsB (MP654), bcsB mgtC (MP655), bcsA_R700D (MP518), bcsA_R700D mgtC (MP520), p_phoP::adrA attTn7 (MP269), and p_phoP::adrA attTn7 bcsA (MP291) Salmonella strains and of the bcsA mgtC (MP142) mutant harboring a plasmid expressing the bcsA gene (pBcsA) or the vector control (pUHE21-2lacI^q) at 18 h postinfection. Error bars represent SDs. ***P < 0.001, ****P < 0.0001, two-tailed t test. Results are representative of at least four independent experiments. (B) Replication within J774A.1 macrophages of WT (14028s), mgtC (EL4), proC (EL605), proC bcsA (MP358), atpB (EL515), and atpB bcsA (MP715) at 18 h postinfection. Error bars represent SDs. ***P < 0.001, ****P < 0.0001, two-tailed t test. Results are representative of two independent experiments. (C) Cellulose biosynthesis by strain p_phoP::adrA attTn7 (MP269) inside macrophages. (D) ATP levels of WT (14028s), mgtC (EL4), p_phoP::adrA attTn7 (MP269), and bcsA p_phoP::adrA attTn7 (MP291) Salmonella strains after 8 h of growth in low-Mg²⁺ medium. Results are representative of two independent experiments.