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. 2015 Apr 6;112(16):5023–5028. doi: 10.1073/pnas.1424044112

Fig. 4.

Fig. 4.

snoRNAs activate PKR in vitro. (A) Autophosphorylation assay with representative snoRNAs. snoRNA concentrations were 10, 50, 100, 200, and 500 nM; perfectly duplexed RNA 106 bp in length (ds106) concentrations were 5 and 10 nM. Phosphorylation activities were normalized with reactions of 5 nM of ds106. Averaged activation data (as assayed in A) for snoRNAs identified (B) or not identified (C) by RIP-Seq. (D) Comparison of autophosphorylation for snoRNAs with different 5′ termini. snoRNA concentrations as in A. (E) Averaged activation data for SNORA3, SNORA71, and SNORD113 with different 5′ termini. mSNORA and hSNORA indicate mouse and human box H/ACA snoRNA, respectively; mSNORD, mouse box C/D snoRNA. Error bars, mean ± SEM, n ≥ 3, except for mSNORD89, mSNORD7 (C), and 5′-OH mSNORD113 (E), which show mean ± SD, n = 2. White spaces indicate where intervening lanes were removed from the figures.