Skip to main content
. 2015 Apr 6;112(16):5023–5028. doi: 10.1073/pnas.1424044112

Fig. 5.

Fig. 5.

snoRNAs activate PKR in cells and in lysate. (A) Quantitation of phospho-PKR (p-PKR) activity. PKR activity was calculated per copy number of the transfected snoRNA, as determined from RT-qPCR of transfected cells. PKR activity values per 1011 RNA copy numbers for 5′-monophosphorylated (p) and 5′-triphosphorylated (ppp) snoRNAs are shown. Dark gray bars are graphed relative to the left y axis, and light gray bars are graphed relative to the right y axis. (B) Western blot of p-eIF2α in PKRWT or PKR−/− lysates, Mock-treated (−), 2 μM 5′-ppp-mSNORA71–treated (+) or 5′-ppp-mSNORD113–treated (1.2, 3.2, and 6.4 μM). Quantification of p-eIF2α activity in MEFs (C) and CHO (D) lysates after treating with 5′-ppp-mSNORA71. Fold change in phospho-eIF2α activity is graphed relative to mock-treated lysate. Error bars, mean ± SEM, n = 3. *P < 0.05.