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. 2015 Apr 6;112(16):5165–5170. doi: 10.1073/pnas.1420308112

Fig. 5.

Fig. 5.

CARDS TX binds PC, DPPC, and SM specifically over other membrane lipids. (A) ELISA and (B) dot blot analyses of CARDS TX binding to a panel of diverse membrane lipids and their components as described in Methods. Full-length, D1, and D2+D3 constructs were tested. The 16 lipids/lipid components on each strip are (1) triglyceride, (2) diacylglycerol, (3) phosphatidic acid, (4) phosphatidylserine, (5) phosphatidylethanolamine, (6) PC, (7) phosphatidylglycerol, (8) cardiolipin, (9) phosphatidylinositol, (10) phosphatidylinositol 4-phosphate, (11) phosphatidylinositol 4,5-bisphosphate, (12) phosphatidylinositol 3,4,5-trisphosphate, (13) cholesterol, (14) SM, (15) 3-sulfogalactosylceramide, and (16) blank.