Fig. 5.
TREX1D18N/D18N mice produce α-dsDNA antibodies, form glomerular immunocomplexes, and have activated adaptive immune responses. (A) Total IgG ELISA (10–12 animals per genotype). (B) Total α-dsDNA antibody ELISA (10–12 animals per genotype). (C) Immunofluorescence (IF) for IgG in frozen kidney sections. (Bar, 50 µm.) Splenocytes were labeled for (D) CD4, CD8, and B220; (E) CD138; (F) CD4, CD69, and CD62L; (G) CD8, CD69, and CD62L; or (H) CD4, CD25, and FoxP3 and enumerated by flow cytometry. *P < 0.05; **P < 0.001; bars represent the mean, and error bars represent the SEM.