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. 2014 Jun 4;6(7):1961–1968. doi: 10.1002/cctc.201402125

Figure 2.

Figure 2

Chimeric fibrils of AP and HRP display a similar fibril core structure as WT Ure2. A) Negative-staining TEM images of WT Ure2 and chimeric protein fibrils after proteinase K (PK) digestion. All scale bars 200 nm. B) Anti-Ure2 Western blot (lower panel) of insoluble pellets of WT Ure2 and chimeric protein fibrils with or without PK digestion, centrifuged and washed with buffer three times, and dissolved in 8 m urea prior to SDS-PAGE (upper panel). The similarity in widths and morphologies of the fibril cores, and immunospecificity to Ure2 antibody of digested chimeric fibrils, suggests that the chimeric fibrils and WT Ure2 share a common fibril core.