Figure 5.

Application of AP chimeric fibrils in a molecular biology assay. Plasmids (as indicated) treated with buffer (Con, control), SAP, Ure2₁₋₉₃-AP fibrils (F), or recycled Ure2₁₋₉₃-AP fibrils (R-F) were transformed into Top10 cells for blue/white selection. White colonies on the plates indicate successful phosphatase treatment and gene insertion. The proportion of white colonies after treatment is represented in the graph. Ure2₁₋₉₃-AP fibrils are at least as effective as SAP but more convenient to use and can be recycled. Furthermore, for the pHSG299 plasmid treated with recycled Ure2₁₋₉₃-AP fibrils, the absence of any colonies growing on the Amp⁺ plate indicates that plasmid contamination of reused Ure2₁₋₉₃-AP fibrils is absent. See Experimental Section for further details.