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. 2015 Apr 1;4(4):56–64. doi: 10.1302/2046-3758.44.2000310

Figs. 10a - 10d.

Figs. 10a - 10d

Figs. 10a - 10d

Figs. 10a - 10d

Figs. 10a - 10d

Histological observation in the experimental group 24 weeks post-operatively, observed using an optical microscope, showing a) cells arranged in a disorderly manner: the scaffold has degraded (Haematoxylin-eosin staining, × 400), b) cells arranged in a disorderly manner, the scaffold has degraded (Alcian blue, × 400), c) the scaffold has mostly degraded, type II collagen in chondrocyte plasma and extracellular matrix is stained a browny-yellow colour (Immunohistochemistry, × 100) and d) the upper layer (red) is cartilage matrix, the middle is the osteochondral interface, and the tidal line is unclear (Safranin O staining, ×40).