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Levels of Th cells stimulated with vaccine candidates assessed with the C-ImmSim server. Simulation with C-ImmSim was performed for each vaccine candidate with one and three immunizations, and Th1 cells stimulated values per microliter were identified 80 days after the first immunization. The best stimulation was induced by PE_PGRS49 protein followed by PE_PGRS 56, PE26, PPE65, and PBP1 proteins.
