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. 2015 Apr 29;16(1):343. doi: 10.1186/s12864-015-1561-x

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© Bok et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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A diagram shows the workflow of cryptic SM production. High molecular weight genomic DNA from A. terreus was mechanically sheared and cloned into shuttle AMAI-BAC (or FAC) clones, SM FACs were selected and transformed into A. nidulans, transformants screened for metabolites followed by compound identification and structure elucidation.