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. 2015 Apr 24;14:60. doi: 10.1186/s12934-015-0246-0

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© Trikka et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Carotenoid production of improved haploid strains. (A) Color formation in yeast cultures due to carotenoids formed in liquid cultures of two independent cultures of AM233 versus control wild type BY4741 cells; (B) wild type EG60, AM228 (rox1), AM229 (rox1, dos2), AM230 (rox1, dos2, yer134c) and AM231 (rox1, dos2, yer134c, vba5), AM233 (rox1, dos2, yer134c, vba5, ynr063w) expressing crtYB/crtI/crtE were grown in independent triplicate cultures and carotenoids were extracted and quantified. Tandem deletions lead to substantial yield improvements.