Table 2.
List of developed yeast strains
| Strain | Genotype | Plasmid description | Source |
|---|---|---|---|
| BY4741 | MATa, his3Δ1, leu2Δ0, met15Δ0, ura3Δ0. | Research Genetics | |
| EG60 | MATα, ura3, trp1, his3. | Erica Golemis | |
| EG60-2 | MATα, ura3, trp1, his3. | YEplac195-YB/I/E (PTDH3-crtYB-tCYC1, PTDH3-crtI-tCYC1, PTDH3-crtE-tCYC1, URA3, 2 μ) | This study |
| EG60-3 | MATα, trp1, his3 PTDH3-crtYB-tCYC1, PTDH3-crtI-tCYC1, PTDH3-crtE-tCYC1, URA3 | This study | |
| EG60-4 | MATα, ura3, trp1, his3. | pUTDH/CLS-ERG20 (F96C) (PTDH3-CcCLS-ERG20 (F96C)-tCYC1, 2 μ, URA3) | This study |
| EG60-5 | MATα, ura3, trp1, his3. | pUTDH3/HMG1-TM-EYFP, 2 μ PTDH3-HMG1-TM-EYFP URA3 | This study |
| EG60-6 | MATα, ura3, trp1, his3. | pUTDH3/Sf126-EYFP, 2 μ PTDH3-Sf126-EYFP URA3 | This study |
| AM228 | MATα his3, ura3, trp1, rox1 derived from EG60 | This study | |
| ΑΜ228-1 | MATα his3, ura3, trp1, rox1 derived from EG60 | YEplac195-YB/I/E (PTDH3-crtYB-tCYC1, PTDH3-crtI-tCYC1, PTDH3-crtE-tCYC1, URA3, 2 μ) | This study |
| ΑΜ228-2 | MATα his3, ura3, trp1, rox1 derived from EG60 | pUTDH/CLS-ERG20 (F96C) (PTDH3-CcCLS-ERG20 (F96C)-tCYC1, 2 μ, URA3) | This study |
| AM229 | MATα his3, ura3, trp1, rox1, dos2 derived from AM228 | This study | |
| AM229-1 | MATα his3, ura3, trp1, rox1, dos2 derived from AM228 | YEplac195-YB/I/E (PTDH3-crtYB-tCYC1, PTDH3-crtI-tCYC1, PTDH3-crtE-tCYC1, URA3, 2 μ) | This study |
| AM229-2 | MATα his3, ura3, trp1, rox1, dos2 derived from AM228 | pUTDH/CLS-ERG20 (F96C) (PTDH3-CcCLS-ERG20 (F96C)-tCYC1, 2 μ, URA3) | This study |
| AM230 | MATα his3, ura3, trp1, rox1, dos2, yer134c derived from AM229 | This study | |
| AM230-1 | MATα his3, ura3, trp1, rox1, dos2, yer134c derived from AM229 | YEplac195-YB/I/E (PTDH3-crtYB-tCYC1, PTDH3-crtI-tCYC1, PTDH3-crtE-tCYC1, URA3, 2 μ) | This study |
| AM230-2 | MATα his3, ura3, trp1, rox1, dos2, yer134c derived from AM229 | pUTDH/CLS-ERG20 (F96C) (PTDH3-CcCLS-ERG20 (F96C)-tCYC1, 2 μ, URA3) | This study |
| AM231 | MATα his3, ura3, trp1, rox1, dos2, yer134c, vba5 derived from AM230 | This study | |
| AM231-1 | MATα his3, ura3, trp1, rox1, dos2, yer134c, vba5 derived from AM230 | YEplac195-YB/I/E (PTDH3-crtYB-tCYC1, PTDH3-crtI-tCYC1, PTDH3-crtE-tCYC1, URA3, 2 μ) | This study |
| AM231-2 | MATα his3, ura3, trp1, rox1, dos2, yer134c, vba5 derived from AM230 | pUTDH/CLS-ERG20 (F96C) (PTDH3-CcCLS-ERG20 (F96C)-tCYC1, 2 μ, URA3) | This study |
| AM233 | MATα his3, ura3, trp1, rox1, dos2, yer134c, vba5, ynr063w derived from AM231 | This study | |
| AM233-1 | MATα his3, ura3, trp1, rox1, dos2, yer134c, vba5, ynr063w derived from AM231 | YEplac195-YB/I/E (PTDH3-crtYB-tCYC1, PTDH3-crtI-tCYC1, PTDH3-crtE-tCYC1, URA3, 2 μ) | This study |
| AM233-2 | MATα his3, ura3, trp1, rox1, dos2, yer134c, vba5, ynr063w derived from AM231 | pUTDH/CLS-ERG20 (F96C) (PTDH3-CcCLS-ERG20 (F96C)-tCYC1, 2 μ, URA3) | This study |
| AM238 | MATα his3, ura3, trp1, rox1, dos2, yer134c, vba5, ynr063w, ygr259c derived from AM233 | This study | |
| AM238-1 | MATα his3, ura3, trp1, rox1, dos2, yer134c, vba5, ynr063w, ygr259c derived from AM233 | YEplac195-YB/I/E (PTDH3-crtYB-tCYC1, PTDH3-crtI-tCYC1, PTDH3-crtE-tCYC1, URA3, 2 μ) | This study |
| AM238-2 | MATα his3, ura3, trp1, rox1, dos2, yer134c, vba5, ynr063w, ygr259c derived from AM233 | pUTDH/CLS-ERG20 (F96C) (PTDH3-CcCLS-ERG20 (F96C)-tCYC1, 2 μ, URA3) | This study |
| AM238-3 | MATα his3, ura3, trp1, rox1, dos2, yer134c, vba5, ynr063w, ygr259c derived from AM233 | pUTDH/CLS-ERG20 (F96C) (PTDH3-CcCLS-ERG20 (F96C)-tCYC1, 2 μ, URA3); pHTDH/CD-HMG2 (PTDH3-CD-HMG2-tCYC1, 2 μ, HIS3; pWTDH/SCLSmat (PTDH3-SCLS-tCYC1, 2 μ TRP1 | This study |
| AM238-4 | MATα his3, ura3, trp1, rox1, dos2, yer134c, vba5, ynr063w, ygr259c derived from AM233 | pUTDH3/HMG1-TM-EYFP, 2 μ PTDH3-HMG1-TM-EYFP URA3 | This study |
| AM238-5 | MATα his3, ura3, trp1, rox1, dos2, yer134c, vba5, ynr063w, ygr259c derived from AM233 | pUTDH3/Sf126-EYFP, 2 μ PTDH3-Sf126-EYFP URA3 | This study |
| AM109-1 | MATa/α, GALp-(K6R)HMG2::HOX2, ura3, his3, trp1, PTDH3-HMG2(K6R)X2-::leu2, PTDH3-HMG2(K6R)::HO1, ERG9/erg9, UBC7/ubc7, ssm4/SSM4, pho86/PHO86, derived from AM102 | pUTDH3/HMG1-TM-EYFP, 2 μ PTDH3-HMG1-TM-EYFP URA3 | This study |
| AM109-2 | MATa/α, GALp-(K6R)HMG2::HOX2, ura3, his3, trp1, PTDH3-HMG2(K6R)X2-::leu2, PTDH3-HMG2(K6R)::HO1, ERG9/erg9, UBC7/ubc7, ssm4/SSM4, pho86/PHO86, derived from AM102 | pUTDH/CLS-ERG20 (F96C) (PTDH3-CcCLS-ERG20 (F96C)-tCYC1, 2 μ, URA3) | This study |