Table 1. Rat primer sequences used for qRT-PCR.
Gene | Sense primer | Antisense primer | PCR product (bp) |
IL-6 | CACAAGTCCGGAGAGGAGAC | ACAGTGCATCATCGCTGTTC | 168 |
IL-1β | CTGTGACTCGTGGGATGATG | GGGATTTTGTCGTTGCTTGT | 210 |
ICAM-1 | ACGCAGTCCTCGGCTTCTG | GGTTCTTGCCCACCTGCTG | 97 |
VCAM-1 | ACAAAACGCTCGCTCAGATT | GTCCATGGTCAGAACGGACT | 152 |
Ccl2 (MCP-1) | ATGCAGTTAATGCCCCACT | TTCCTTATTGGGGTCAGCAC | 167 |
Ccl3 (MIP-1α) | TGCCCTTGCTGTTCTTCTCT | AAAGGCTGCTGGTCTCAAAA | 152 |
GAPDH | GCAAGTTCAACGGCACAG | GCCAGTAGACTCCACGACAT | 140 |
PCR: Polymerase chain reaction; ICAM-1: Intercellular cell adhesion molecule-1; VCAM-1: Vascular cell adhesion molecule-1; MCP-1: Monocyte chemotactic protein-1; MIP-1α: Macrophage inflammatory protein-1α.