Figure 2. Brain metastasis after cardiac ventrical injection of high brain-metastatic variants.

4T1-RFP cells were injected into the left cardiac ventricle of female nude mice. (A) Intact brain with metastases. (B) Metastases were located in both cerebral hemispheres. (C) H&E stain demonstrates thrombosis-like infarction of the brain parenchyma (agnification 200x). (D) Intravascular metastases appears to follow blood vessels (confocal fluorescence microscopy). Bar = 100 μm.