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. 2014 Dec 30;6(5):2623–2640. doi: 10.18632/oncotarget.3098

Figure 9. Effects of MDM2 overexpression and knockdown on JapA-induced cell death.

Figure 9

(A, B) MDA-MB-231 cells were transfected with a Myc plasmid or a Myc-MDM2 plasmid for 24 h and then treated with various concentrations of JapA for (A) 24 h for the expression levels of Myc-MDM2 and p53, where Myc-MDM2 and p53 were detected by Western blotting using antibodies against Myc and p53, respectively; and (B) 72 h for determination of the cell viability. (C, D) MDA-MB-231 cells were transfected with MDM2 siRNA or the respective control siRNA for 36 h and then treated with various concentrations of JapA for (C) 24 h for expression levels of MDM2 and p53, where MDM2 and p53 were detected by Western blotting using antibodies against MDM2 and p53, respectively; and (D) 72 h for determination of the cell viability. All assays were performed in triplicate.

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