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. 2015 Jan 8;6(5):2654–2666. doi: 10.18632/oncotarget.3042

Figure 4. Inhibition of STAT3 activity and depletion of Mcl-1 sensitize radioresistant laryngeal HEp-2 cells.

Figure 4

(A and B) RR-HEp-2 cells were untreated (Ctrl) or treated with the indicated dose of radiation in the absence (Ctrl) or presence of 100 μM S3I-201 and then incubated for 48 h. (C) RR-HEp-2 cells were treated with each dose of radiation as indicated in the absence or presence of 50 μM S3I-201. High cytotoxicity reduced 100 μM S3I-201 to 50 μM in clonogenic assay. (D-F) RR-HEp-2 cells were transfected with 100 nM control siRNA or Mcl-1 siRNA. After 48 h, the cells were treated with each dose of radiation. (C and F) The clonogenic survival fraction was determined by clonogenic assay. Cell viability was determined by FACScan flow cytometry, and data are presented as the percentage of PI-positive cells (B and E). Cells were analyzed by immunoblotting with the indicated antibodies. β-actin was used as a loading control. The data represent typical results and are presented as mean ± standard deviation of 4 independent experiments; *P < 0.05 compared with irradiated respective control cells.