Figure 3. Co-inhibition of PDGFRβ and c-MYC suppresses MB cell proliferation and migration.

MB cells were transfected with gene-specific siRNAs for PDGFRβ and c-MYC and also with PDGFR and c-MYC specific inhibitors alone or in combination for 48 h. (A) Confirmation of specific gene knockdown by Western blotting analysis. β-actin was used as the loading control. (B) The effects of c-MYC inhibitor 10058-F4 on MB cell proliferation. (C) The effects of siRNAs and inhibitors on MB cell proliferation were determined using MTS. *p < 0.05, **p < 0.01 (paired t-test, sample vs. control). (D) The effects of co-inhibiting PDGFRβ and c-MYC on MB cell migration. Daoy cells were transfected with gene specific siRNAs for PDGFRβ and c-MYC and also with PDGFR and c-MYC specific inhibitors alone or in combination for 36 h. Treated cells were then detached and re-distributed in equal amounts in a 48-well plate before a linear wound was made. The image was captured immediately after that an artificial wound was made at 0^th h and also at 24^th h (Figures S3a, S3b). Quantified results were calculated from the images. Percentage wound closure shows the migration rate in PDGFRβ^KD, c-MYC^KD or PDGFRβ^KDc-MYC^KD cells when compared to control sample, *p < 0.05, **p < 0.01, ***p < 0.001 (paired t-test, sample vs. control).