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. 2014 Oct 21;6(5):2725–2735. doi: 10.18632/oncotarget.2614

Figure 2. Characterization of mouse endocan.

Figure 2

Figure 2

(A) Immunoprecipitation and western blot of HEK293 cells overexpressing recombinant mouse endocan, EL-4 cell supernatant, (B) and sera from 4 mouse strains (SCID, 129Sv, Balb/C and C57Bl6). Samples were analyzed under non reducing condition. (C) Sequence comparison between human and mouse endocan. (D) Immunoprecipitation and western blot of HEK293 cell supernatants overexpressing wild type mouse endocan, mutant S136M, and wild type human endocan, mutant M135S, (E, F) chimaeras constructions of exon 1, (G,H) chimaeras constructions of exon 2 and (I, J) chimaeras constructions of exon 3.