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. 2014 Dec 26;6(5):2843–2855. doi: 10.18632/oncotarget.3066

Figure 3. MIR196B regulates FAS-mediated apoptosis in SW480 cells.

Figure 3

(A) Western blot analyses of FAS, FasL, and FAS-mediated molecules in MIR196B- or anti-MIR196B-transfected SW480 cells. Proteins were extracted 72 h after transfection for western blot analysis. The data shown were normalized to GAPDH levels and are presented as fold-change in MIR196B- or anti-MIR196B-transfected cells relative to mock-transfected cells. The experiment was repeated four times. (B) Western blot analyses of FAS and FAS-mediated molecules in FAS mAb-treated or MIR196B-transfected SW480 cells. (C) Western blot analyses of FasL, NF-κB, and ERK2 in FAS mAb-treated or MIR196B-transfected SW480 cells. Protein was extracted 72 h after MIR196B transfection or FAS mAb treatment or 48 h after MIR196B transfection into cells cultured with FAS mAb for 24 h. The data shown were normalized to GAPDH levels and are presented as the fold-change in MIR196B-transfected or FAS mAb-treated cells relative to mock-treated cells. Data assessed from four independent experiments and the P values were calculated by t-test (* P < 0.05; ** P < 0.01; ns = not significant).