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. 2014 Dec 26;6(5):2843–2855. doi: 10.18632/oncotarget.3066

Figure 4. MIR196B regulates FAS-mediated apoptosis in SW480 or HT29 cells.

Figure 4

(A) Western blot analyses of FAS, FasL, and FAS-mediated molecules in MIR196B- or anti-MIR196B-transfected HT29 cells. Experiments were repeated four times and the P values were calculated by t-test (* P < 0.05; ** P < 0.01; ns = not significant). (B) Flow cytometry analysis of FAS-mediated apoptosis in MIR196B- or anti-MIR196B-transfected SW480 cells. Cells were cultured in the absence (upper panel) or presence (lower panel) of FAS mAb, stained with annexin V and PI, and analyzed by flow cytometry. The number in each box indicates the percentage of annexin V- and/or PI-positive cells. (C) Flow cytometry analysis of apoptosis in MIR196B- or anti-MIR196B-transfected HT29 cells. Cells were cultured in the absence (upper panel) or presence (lower panel) of 5-FU. Cells were collected 72 h after transfection, stained with annexin V and PI, and analyzed by flow cytometry. The number in each box indicates the percentage of annexin V- and/or PI-positive cells. Experiments were repeated three times with duplicates.