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. 2015 Jan 22;6(5):2928–2938. doi: 10.18632/oncotarget.2758

Figure 2. EZH2 GOF catalytic activity inhibits cell contractility and is necessary for the maintenance of 3D-branching morphology.

Figure 2

(A) Western blot analysis of A375 stable cells cultured on top of ECM (4 days) or tissue-culture treated plastic. pMLC is a marker of cell contractility and ROCK activity. (B) A375 Vector, EZH2 Y641F or EZH2 Y641N cells were seeded on top of ECM and simultaneously treated with 10 μM Y-27632 (ROCK inhibitor). Western blot analysis (top) was performed 4-days post-seeding/treatment. Images (10x, bottom) were captured 2 days post-seeding/treatment. (C and D) A375 stable cell lines were treated with DMSO or 1 μM GSK126 for 7 days in 2D culture and re-plated (and re-treated) on top of ECM and imaged (C, 10x) or lysed for western blot analysis (D) 4 days post-seeding.