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. 2014 Dec 26;6(5):3013–3032. doi: 10.18632/oncotarget.3072

Figure 3. Knockdown of PRMT7 in invasive breast cancer cells inhibits their ability to invade.

Figure 3

PRMT7 was stably depleted in MDA-MB-231 and BT549 using lentiviral delivery of a PRMT7-targeting shRNA. A non-targeting shRNA was used as a control (shControl). Western analysis for PRMT7 in stably depleted MDA-MB-231 and BT549 cells (A). Tubulin serves as a loading control. Densitometry of the band intensities are indicated below in parentheses. MDA-MB-231 and BT549 cells depleted of PRMT7 were assessed for effects on motility and invasion. Cells were plated at equal numbers into Transwell chambers and incubated for 24 h. Motility was analyzed using Transwell chambers without a Matrigel (Motility). Invasion was analyzed using Transwell chambers containing a Matrigel layer (Invasion). Representative images of cells that have passed through the Transwell chamber ± Matrigel at 40X magnification (B). MDA-MB-231 and BT549 cell numbers that passed through the Transwell chambers in the absence of Matrigel (C and E, respectively: motile cells/field) or in the presence of Matrigel (D, MDA-MB-231 and F, BT549: invasive cells/field) were determined. Data represents the mean ± standard error of four independent experiments for MDA-MB-231 and three independent experiments for BT549 (*p < 0.05, **p < 0.01 comparing to control).