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. 2014 Dec 26;6(5):3013–3032. doi: 10.18632/oncotarget.3072

Figure 4. Overexpression of PRMT7 in non-invasive breast cancer cells promotes invasion.

Figure 4

PRMT7 was stably overexpressed in MCF7 cells by lentiviral transduction. An empty vector expressing mGFP was used as a control. Total protein lysates from MCF7 cells stably expressing empty vector, PRMT7-MycDDK or PRMT7-GFP were analyzed by Western blotting for PRMT7 expression (A). Tubulin serves as a loading control. B, Fluorescence images (40X) of MCF7 cells stably expressing an empty vector or PRMT7-GFP. MCF7 cells stably expressing PRMT7-MycDDK or PRMT7-GFP were analyzed for motility and invasion using Transwell chambers as previously described. However, cells that crossed the chamber membrane were counted following a 72 h incubation. Representative images of cells that have passed through the Transwell chamber ± Matrigel at 40X magnification (C). Cells that passed through the chamber membranes without a Matrigel layer (D: motile cells/field) or containing a Matrigel layer (E: invasive cells/field) were counted. Data represents the mean ± standard error of eleven independent experiments for PRMT7-MycDDK and nine independent experiments for PRMT7-GFP (*p < 0.05, **p < 0.01 comparing to Empty vector control).