Figure 1. KITENIN expression in human glioma samples.
(A) Western blotting analysis showing that the overall expression of KITENIN tended to increase along the sequence from normal brain tissues to low-grade gliomas (WHO grades I-II), and then to high-grade gliomas (WHO grades III-IV). (B) qRT-PCR showing that the levels of expression of KITENIN mRNA were significantly higher in glioma tissues than in normal brain tissues (P=0.010). (C) Relative KITENIN mRNA levels, normalized to GAPDH mRNA levels, were significantly higher in tumor than in normal tissue (P=0.037). (D) Immunohistochemistry with anti-KITENIN antibody on human glioma tissue samples showing cytoplasmic positivity, with representative figures according to staining intensity. (E) KITENIN expression, represented by the staining intensity from WHO tumor grade I through to grade IV, showing statistically significant differences between tumor grade groups (P=0.001, Table 5). The bar graphs show the mean ± the standard error of the mean (SEM) for each group. (*P<0.05, **P<0.01).
