Figure 4. Expression changes of IFIT3 mRNA depending on stimulation with IFN and blockade of NFκB and STAT1 signaling.
Quantitative PCRs were performed on COLO357FG (A) and L3.6pl (B) cells. Bars represent means of triplicate experiments with error bars representing SD. Expression changes are shown as fold-changes as compared to unstimulated cells (bar #1 in each graph). Cells were either treated with IFN-α, BAY 11-7082 (to block NFκB signaling), or S 14-95 (to block STAT1 signaling) or any combination of the three. Statistical analysis was performed using unpaired t-tests.