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. 2014 Sep 17;6(5):3306–3318. doi: 10.18632/oncotarget.2494

Figure 6. Effects of IFIT3 and SOX9 overexpression on secretion levels of the inflammatory cytokines Il-6 and TNF-α.

Figure 6

(A) ELISA assessing the concentrations of Il-6 in supernatants of COLO357FG/CMV-null, COLO357/CMV-IFIT3 and L3.6/CMV-null without (black bars) or with (grey bars) previous IFN-α treatment. (C) The graph shows results of ELISAs on the same cell lines assessing TNF-α concentrations but without IFN treatment. (B&D) ELISAs of IL-6 (B) and TNF-α (D) concentrations were performed on the supernatant of L3.6pl-6TR-SOX9shRNA. Tetracycline can be used to induce silencing of SOX9 by SOX9shRNA (striped bars). IL-6 concentration was assessed also depending on IFN-α treatment of the cells (grey bars). Bars in all graphs represent mean values of triplicate experiments with error bars representing the SD. All values are shown as fold-changes compared to the respective concentrations measured in untreated COLO357FG/CMV-null (A/C) and L3.6pl-6TR-SOX9shRNA cells (B/D), respectively (bars #1 in each graph). Statistical significance was tested using unpaired t-tests.