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. 2014 Dec 18;6(5):3375–3393. doi: 10.18632/oncotarget.3270

Figure 2. KDELR stimulation by Bodipy-KDEL promotes ECM degradation.

Figure 2

(A) A375MM cells were grown on rhodamine-conjugated crosslinked gelatine (red) for 16 h in the presence of BB94. Following BB94 wash-out, the cells were incubated for a further 3 h with the membrane permeant KDELR agonist Bodipy-KDEL (3 μM) or the control peptide Bodipy-KDEA (3 μM) (as indicated). As an additional control, the cells were incubated with vehicle alone (Vehicle). After fixing, the cells were stained with phalloidin (green). Merged images of red and green signals are also shown (Merge). Invadopodia are shown in the enlargements of the boxed regions (small right panels: green and red signals). Scale bars, 10 μm. The images are representative of three independent experiments. (B) Quantification of the degradation area per cell. Data are degradation area per cell (% of control), as means ±SEM of three independent experiments, with at least 100 cells quantified per experiment. ** p<0.01, compared to Vehicle cells (t-test).