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. 2014 Oct 25;6(5):3394–3408. doi: 10.18632/oncotarget.2583

Figure 4. Combined treatment induces apoptosis.

Figure 4

(A) Analysis of DNA content by FACS after 24 and 48 hrs of treatment with the two drugs either singly or combined. Percentages of cell cycle phases (G1-S-G2/M) are included in the figure. The asterisk (*) points the sub-G1 population in the combined samples. (B) Activation of caspase-3 by enzymatic assay in JeKo-1 cells 24, 48, 72 and 120 hrs after treatment with PF-00477736 and MK-1775 either singly or combined. Data are represented as fold change over untreated cells and are the mean ±SD of two independent experiments. (C) TUNEL assay performed in JeKo-1 cells 72 hrs after treatment with the drugs singly or combined.