Table 2.
Primer sequences used in reverse transcription quantitative PCR analysis.
| Gene | Forward 5′–3′ | Reverse 5′–3′ | Expected product length (bp) |
|---|---|---|---|
| PaNPR1 | TGGCTTATCAGTGCTTGCTC | CCTCCTTATCCTCGTTGTATGC | 119 |
| PaNPR2 | GAACCACTACTAGGAGAAG | TTGCCAGACTAACTCTAC | 97 |
| PaNPR3 | CTTCCCGACTTATTCTACCTTGAG | CGATCTGCTGTACTCCTTGTC | 126 |
| PaNPR4 | AGGTGCTGCTGCTGCTAC | TGGATTCGTGGCTTCTCTATGC | 94 |
| PaNPR5 | GTCGAACAGTTGGCATTG | GAGCACTTTCATCACATCTTC | 84 |
| PaPR1 | GCGGCTGGAAAGGTTTGT | GGGGCTGTAGTTGCAAGT | 102 |
Primer sequences were designed to amplify fragments no larger than 150bp for each of the five NPR1-like genes identified in P. americana in order to perform RT-qPCR analysis.