Table 3.
Reverse transcription quantitative PCR optimization.
| Gene | T | E | R | LDR | M1 | M2 |
|---|---|---|---|---|---|---|
| 18S | 58C | 108.2 | 0.995 | 1:9–1:729 | 0.0185 | 0.0579 |
| -1 tubulin | 58C | 91.8 | 0.997 | 1:3–1:729 | 0.0064 | 0.2076 |
| Actin | 58C | 101.7 | 0.995 | 1:9–1:729 | 0.0671 | 0.0512 |
| PaNPR1 | 56C | 95.6 | 0.996 | 1:3–1:729 | ||
| PaNPR2 | 59C | 93.2 | 0.991 | 1:3–1:729 | ||
| PaNPR3 | 63C | 96.0 | 0.992 | 1:3–1:729 | ||
| PaNPR4 | 63C | 96.9 | 0.996 | 1:3–1:729 | ||
| PaNPR5 | 62.5C | 93.1 | 0.991 | 1:3–1:729 | ||
| PaPR1 | 58C | 97.6 | 0.992 | 1:3–1:729 |
Primer sets of the five NPR1-like genes and PR1 from P. americana as well as endogenous control genes were optimized for annealing temperatures (T) that yielded sufficient efficiency (E) and coefficient of determination (R) values. Linear dynamic range (LDR) indicates the minimum and maximum dilutions used to create a calibration curve. The stability (M-value) of the reference genes is also indicated for SA, MeJA, and Phytophthora cinnamomi treated (M1) and different tissue samples (M2).