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Development of podocyte-specific EGFR deletion mice. (A) Schematic for the generation of EGFRpodKO mice by crossing Podocin.Cre mice with EGFRflox/flox mice. (B) EGFR deletion of exon 3 and Cre expression are verified by PCR using glomerular genomic DNA as the template. (C) EGFR attenuation in podocytes is confirmed by immunoblotting of isolated glomerular lysates. Data are representative of three separate experiments (n=5–7 mice/group).
