Figure 2.
Proteinuria and podocyte loss are attenuated in mice with selective deletion of EGFR in podocytes. (A) EGFRpodKO mice aged 9–10 weeks and their control littermates are subjected to five consecutive STZ injections. Mouse urine is collected for 24 hours and creatinine and albumin are measured at 32 weeks after STZ injection (n=5–7 per group). (B) Representative micrographs of electron microscopy from different groups of mouse kidney (n=3–5 per group). (C) Representative kidney sections are stained with the antibody against WT1. (D) Podocyte numbers are counted randomly in 15 glomeruli/section (n=5–7 per group). (E) Immunoblotting of isolated glomeruli lysates from EGFRpodKO mice and their control littermates 32 weeks after STZ injection are analyzed with the indicated antibodies (n=5–7 mice/group). (F) Densitometry of the data in E. Data are presented as the mean±SEM. *P<0.05; **P<0.001. Veh, vehicle; STZ, streptozocin.