Figure 3.
Deletion of EGFR in podocytes attenuates ERK activation, TGFβ-Smad2/3 signaling activation, and fibronectin expression, but not Src phosphorylation, in diabetic kidney glomeruli. (A) Immunoblotting of isolated glomeruli lysates from EGFRpodKO mice and their control littermates 32 weeks after STZ injection are analyzed with indicated antibodies. (B) Densitometry of the data in A. (C) Immunoreactivity in diabetic EGFRpodKO mice and littermate controls with specific antibodies against fibronectin (green), podocyte marker synaptopodin (red), and DAPI (blue) (n=5–7 mice/group). Veh, vehicle; STZ, streptozocin.