Figure 4.

Inhibition of ROS production blunts the signaling pathway alterations in WT diabetic mouse glomeruli. WT 129svj mice aged 9–10 weeks are subjected to five consecutive STZ injections. (A and C) Apocynin (A) or mito-tempol (C) is administered by intraperitoneal injections starting 2 days after STZ injection for 3 weeks. Immunoblotting of isolated glomeruli lysates from control, diabetic mice with or without treatment is performed by using indicated antibodies. (B and D) Densitometry of the data in A and C, respectively (n=5–7 mice/group). Veh, vehicle; STZ, streptozocin.