Figure 6.

Blocking EGFR phosphorylation inhibits the effects of high-glucose treatment on cultured podocytes. (A) With exposure of the differentiated podocytes to high glucose (25 mM) for either 24 or 48 hours, phosphorylation of Src at Y416; EGFR at Y845, Y1173, and Y1068; and Smad2/3 is increased. TGFβ and cleaved caspase3 expression is increased, but Bcl2 expression is inhibited. (B) Densitometry of the data in A. (C) In cultured differentiated podocytes, AG1478 treatment inhibits high-glucose–induced phosphorylation of EGFR and ERK, activation of TGFβ-Smad2/3 signaling, and expression alterations of cleaved caspase3 and Bcl2. (D) Densitometry of the data in C. Results from three separate experiments are shown. Veh, vehicle.