Figure 7.

Blocking ROS production or c-Src kinase activity inhibits the effects of high-glucose treatment on cultured podocytes. (A) Pretreatment of the differentiated podocytes with either apocynin or mito-tempol inhibited phosphorylations of c-Src at Y416 and EGFR at Y845, damped TGFb-Smad2/3 signaling activation and blunted the alterations of cleaved caspase3 and Bcl2 expression in response to high glucose treatment. (B) Densitometry of the data in A. (C) Pretreatment of the differentiated podocytes with PP2 not only inhibited c-Src phosphorylation, but also restricted phosphorylation of EGFR and activation of TGFβ-Smad2/3 signaling pathway in response to high glucose treatment. (D) Densitometry of the data in C. Representative data from three separate experiments are shown. Veh, vehicle.