Figure 8.

Knocking down EGFR expression inhibits the effects of high-glucose treatment on cultured podocytes. (A) In cultured differentiated podocytes, specific EGFR siRNA knocks down expression of EGFR-inhibited high-glucose–induced TGFβ-Smad2/3 signaling activation and alterations of expression of cleaved caspase3 and Bcl2. (B) Densitometry of the data in A. (C) Representative images of phalloidin staining patterns observed in control or EGFR siRNA transfected differentiated podocytes in response to mannitol or glucose treatment. Representative data from three separate experiments are shown. Ctrl, control.