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. 2004 Jun 22;5:15. doi: 10.1186/1471-2350-5-15

Figure 2.

Figure 2

Molecular analysis of the TGF-β1 allele variations at codon 25: (A) representative derivative melting curves of a representative LightCycler run for analysis of the Arg25Pro gene polymorphism in the TGF-β1 gene. Samples shown are: no template control (blue line) Arg25Arg (red line), Arg25Pro (black line), respectively. The amplified products of this LC run were separated in a 1.5% agrose gel showing the expected amplicon 523-bp in size (inlet). (B) Sequence analysis of the Arg25Pro alleles. 80 ng genomic DNA from a patient having a heterozygous allele constellation was taken as template in standard PCR and amplified using primers Arg25Pro-for and Arg25Pro-rev. Amplified DNA was gel purified, cloned into the pGEM-T Easy vector, and sequenced with primer Arg25Pro-for on the sense strand on the ABI PRISM310 Genetic Analyzer. The upper chromatogram was derived from the Arg25-allele, the lower chromatogram from the Pro25-allele. Amino acids Arg25 or Pro25 encoded by the polymorphic site (CGG or CCG) are boxed.