Figure 6.

Effect of prior exposure on female attraction to male urine and conditioned place preference responses. After pre-exposure in the home cage to urine from a wild-stock male, females were tested in a clean arena with four locations marked by visually distinctive floor tiles bearing small petri dishes (A). On the learning day, one dish contained male urine streaks familiar from the home cage treatment, one dish contained urine from a novel male and two dishes contained water streaked as a control. Conditioned place preference tests were conducted after 24 h and 48 h when all dishes contained water streaks. Data show the bias in time spent in urine locations minus the average time spent in control locations (mean ± sem, n = 19 per treatment) among females pre-exposed in the home cage to male urine for 30 min (B) or 7 days (C). Asterisks indicate greater bias towards a urine stimulus location than water controls (*P < 0.05, **P < 0.01, ***P < 0.005, ****P < 0.001).