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Transcriptional analysis by qPCR of innate immune related genes cyp1a, irg1l, il1b, and mmp9 of germ-free zebrafish infected at 5 dpf by 24 h of immersion with different listerial strains. Data are expressed as mean ± SD of four independent experiments (each with 10 embryos pooled for total RNA extraction and qPCR). *P < 0.05, **P < 0.01 and ***P < 0.001.
